Journal: Journal of Traditional Chinese Medical Sciences

Article Title: Exploring the mechanism of Yishen Daluo decoction in the treatment of multiple sclerosis based on network pharmacology and in vitro experiments

doi: 10.1016/j.jtcms.2023.03.002

Figure Lengend Snippet: Fig. 6. Effect of YSDLD on the expression of (A) AKT & p-AKT (B) PI3K & p-PI3K using Western blot analysis. Notes: YSDLD: Yishen Daluo decoction; LPS: lipopolysaccharide; p-AKT: phosphorylation-protein kinase B; AKT: protein kinase B; p-PI3K: phosphorylation-phosphatidylinositol3- kinase; PI3K: phosphatidylinositol3-kinase. #P < .05 vs. the control group; *P < .05 vs. the LPS group.

Article Snippet: At 4 C, the membrane was incubated with primary antibodies overnight (1:1000; rabbit antibodies to phosphorylated (p)-Akt, Akt, phosphorylated (p)-PI3K, PI3K; Cell Signaling Technology, Beverly, MA).

Techniques: Expressing, Western Blot, Phospho-proteomics, Control

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Expression of Signal Transducer and Activator of Transcription 3 and Suppressor of Cytokine Signaling 3 in Urothelial Carcinoma

doi: 10.1016/s1607-551x(09)70569-8

Figure Lengend Snippet: Figure 1. Immunohistochemical staining of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) Tyr705 and cytokine signaling 3 (SOCS3) in urothelial carcinomas. Nuclear staining of p-STAT3 (Tyr705): (A) low intensity grade (insert in A is normal urothelium); (B) high intensity grade. Cytoplasmic staining of SOCS3: (C) low intensity grade; (D) high intensity grade. Original magnification, 400×.

Article Snippet: The membranes were blocked with 5% non-fat dried milk in Tris-buffered Saline (pH 7.4) with Tween-20, and then incubated with either anti-phospho-STAT3 (Tyr705) (1:1,000 dilution; Cell Signaling Technology) or SOCS3 rabbit polyclonal primary antibody (Santa Cruz Biotechnology Inc.).

Techniques: Immunohistochemical staining, Staining

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Expression of Signal Transducer and Activator of Transcription 3 and Suppressor of Cytokine Signaling 3 in Urothelial Carcinoma

doi: 10.1016/s1607-551x(09)70569-8

Figure Lengend Snippet: Figure 2. Western blotting of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) Tyr705 and cytokine signaling 3 (SOCS3) protein in low-grade and high-grade UCs. p-STAT3 (Tyr705) expression was low and high in low- and high-grade UCs, respectively. Lane 1 = low-grade UC; lane 2=high-grade UC. However, the expression of SOCS3 was simi- lar in low- and high-grade UCs. GADPH protein expression was used as an internal control.

Article Snippet: The membranes were blocked with 5% non-fat dried milk in Tris-buffered Saline (pH 7.4) with Tween-20, and then incubated with either anti-phospho-STAT3 (Tyr705) (1:1,000 dilution; Cell Signaling Technology) or SOCS3 rabbit polyclonal primary antibody (Santa Cruz Biotechnology Inc.).

Techniques: Western Blot, Expressing, Control

Journal: British Journal of Nutrition

Article Title: dl-Methionine supplementation in a low-fishmeal diet affects the TOR/S6K pathway by stimulating ASCT2 amino acid transporter and insulin-like growth factor-I in the dorsal muscle of juvenile cobia (Rachycentron canadum)

doi: 10.1017/s0007114519001648

Figure Lengend Snippet: Fig. 2. Total and phosphorylation levels of target of rapamycin (TOR), S6 kinase (S6K) and 4E-binding protein 1 (4E-BP1) were examined by Western blot (A) and quantified (B–D) in the dorsal muscle of cobia fed the 0·72, 1·24, and 1·86 % methionine (Met) diets. Results are represented as means with standard errors (n 3) and were analysed using ANOVA followed by Duncan’s multiple range test. a,b Mean values with unlike letters are significantly different (P < 0·05). GAPDH, glycer- aldehyde-3-phosphate dehydrogenase.

Article Snippet: Appropriate primary antibodies were purchased from Cell Signaling Technology Inc.: phosphor-TOR (Ser2448; rabbit no. 2971), TOR (rabbit no. 2972), phospho-p70-S6K (Ser371; rabbit no. 9208), p70-S6K (rabbit no. 9202), phospho4E-BP1 (Thr37/46; rabbit no. 9459) and 4E-BP1 (rabbit no. 9452).

Techniques: Phospho-proteomics, Binding Assay, Western Blot